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AN EXPERIMENTAL STUDY OF INHIBITORY EFFECT OF LASER IRRADIATION ON ENAMEL DEMINERALIZATION OF INITIAL CARIOUS LESION

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Abstract

°á·Ð
Ãʱ⠿ì½Ä º´¼Ò¿¡¼­ ·¹ÀÌÀú Á¶»ç¿¡ ÀÇÇÑ ¿ì½ÄÁøÇà ¾ïÁ¦È¿°ú¸¦ ±Ô¸íÇϱâ À§ÇØ ÀÚ¿¬Ä¡¸¦ ´ë
»óÀ¸·Î STPP Àΰø¿ì½Ä¿ë¾×À» »ç¿ëÇÏ¿© ¿ì½Äº´¼Ò¸¦ Çü¼ºÇÏ°í ź»ê°¡½º ·¹ÀÌÀú¸¦
10J/cm2, 20J/cm2, 30J/cm2,
40J/cm2, 50J/cm2ÀÇ ¼¼±â·Î Á¶»çÇÑ ÈÄ Ä®½· ¿ëÃâ·®À» ÃøÁ¤ÇÏ¿©
³»»ê¼º Áõ°¡¿¡ °¡Àå È¿°úÀûÀÎ Á¶»ç¼¼±â¸¦ °áÁ¤ÇÑ ´ÙÀ½, Ç¥º»À» ´Ù½Ã ´ëÁ¶±º(ºñ󸮱º), ¥°±º
(·¹ÀÌÀú Á¶»ç±º, 40J/cm2), ¥±±º(ºÒ¼ÒµµÆ÷±º, 1.23% APF) ±×¸®°í ¥²±º(ºÒ¼Òµµ
Æ÷¿Í ·¹ÀÌÀú Á¶»ç º´¿ë±º)À¸·Î ³ª´©¾î °¢°¢ ó¸®ÇÏ°í °è¼ÓÀûÀ¸·Î Àΰø¿ì½ÄÀ» ¾ß±â½ÃŲ ÈÄ
¿ì½Ä¹ý¶ûÁú Ç¥¸éÀÇ ¹Ì¼¼°æµµ¸¦ ÃøÁ¤ÇÏ°í ÁÖ»çÀüÀÚÇö¹Ì°æÀ¸·Î Ç¥¸éº¯È­¸¦ °üÂûÇÏ¿© Ãʱ⠿ì
½Äº´¼ÒÀÇ ÁøÇà¾ïÁ¦ È¿°ú¸¦ Æò°¡Çѹ٠´ÙÀ½°ú °°Àº °á·ÐÀ» ¾ò¾ú´Ù.
1. Ãʱâ Àΰø¿ì½Ä º´¼ÒÀÇ ·¹ÀÌÀú Á¶»ç¿¡ ÀÇÇÑ ³»»ê¼º Áõ°¡´Â 30J/cm2 ÀÌ»ó
¿¡¼­ À¯ÀǼº ÀÖ°Ô Áõ°¡ÇÏ¿´À¸¸ç È¿°úÀûÀÎ Á¶»ç ¼¼±â´Â 40J/cm2¿Í
50J/cm2 ¿´´Ù.
2. ·¹ÀÌÀú Á¶»ç¿¡ ÀÇÇØ Ãʱâ Àΰø¿ì½Ä º´¼ÒÀÇ Ç¥ÃþºÎ(20J/cm2,
50J/cm2¿¡¼­ÀÇ ¿ì½ÄÁøÇàÀÌ ¾ïÁ¦µÇ¾ú´Ù.
3. ·¹ÀÌÀú Á¶»ç±ºÀÇ ¿ì½ÄÁøÇà ¾ïÁ¦ È¿°ú´Â Ç¥Ãþ(20¥ìm)¿¡¼­ ºÒ¼Ò(1.23% APF) µµÆ÷±º ¹×
·¹ÀÌÀú Á¶»ç¿Í ºÒ¼ÒµµÆ÷ º´¿ë±º¿¡ ºñÇØ ¶³¾îÁ³À¸³ª, 50¥ìm ±íÀÌ¿¡¼­´Â 3°³±º ¸ðµÎ °°¾Ò´Ù.
4. ·¹ÀÌÀú Á¶»ç±º(40J/cm2), ºÒ¼Ò(1.23% APP) µµÆ÷±º ±×¸®°í ·¹ÀÌÀú Á¶»ç¿Í
ºÒ¼ÒµµÆ÷ º´¿ë±º ¸ðµÎ 1000m ±íÀÌ À̻󿡼­´Â ¿ì½ÄÁøÇà ¾ïÁ¦È¿°ú¸¦ °üÂûÇÒ ¼ö ¾ø¾ú´Ù.
#ÃÊ·Ï#
The purpose of this study was to evaluate the inhibitory effect on demineralization of
enamel of initial carious lesion by irradiation of laser.
For this study, crowns of extracted human premolar teeth were sectioned sagitally
(Mesio-distla and bucco-lingual) and sound buccal or lingual enamel surfaces were
selected for experimental specimens, and initial carious lesions were produced artificially
using demineralizing solution (STPP, 0.1M Lactic acid containing 0.2mM Sodium
tripolyphosphate, brought pH to 4.2 with adding sodium hydroxide).
For determining the most effective irradiation energy of laser to increase
acid-resistance of enamel, 120 specimens were irradiated with CO2 laser
under condition of energy density with 10J/cm2, 20J/cm2,
30J/cm2, 40J/cm2 and 50J/cm2. All of those
specimens were exposed to 0.5N HCIO4, solution containing 0.5%
LaCl3 for 60 seconds for demineralization. Dissolved calcium and phosphate
concentration in this solution were determined by the atomic absorption and the U-V
spectrophotometer.
For evaluation of the inhibitory effect of laser irradiation on enamel demineralization,
150 specimens were divided into 2 groups to test microhardness (100 specimens) and
observe ultra-structural surface changes (50 specimens) of enamels. It each group, they
were divided again into 5 sub-groups as ACL (Artificial carious lesion), Control (No
treatment), Group I (Laser irradiation only, 40J/cm2), Group ¥± (Fluoride
application only, 1.23%, APF) and Group ¥² (Fluoride appliaction+Laser irradiation). ALL
treated specimens were exposed to STPP solution again for 5 days to produce carious
lesion progressively, and then tested microhardness of exposed surface of enamels with
microhardness tester and observed ultra-structural surface changes with SEM.
The results were as follows.
1. The acid-resistance of initial carious lesions increased significantly when irradiated
30J/cm2 energy and most effective energy of irradiation for increasing
acid-resistance were 40J/cm2 and 50J/cm2.
2. Laser irradiation on initial carious lesion was effective for inhibiting caries
progression at 20¥ìm and 50¥ìm in depth.
3. Group I (Laser irradiation only, 40J/cm2) showed inhibition of caries
progression less effective than that of group ¥±(Fluoride application only, 1.23% APF)
and group ¥²(Fluoride application+Laser irradiation) in 20¥ìm depth but 50¥ìm in depth,
all three groups showed same effect.
4. All groups did not show any inhibitory effect on caries progression under 100¥ìm
in depth.

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